5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Details-enriched characterization of cell populations. Morphological information from illustrations or photos can increase for the richness of flow cytometry information. For instance, the figure exhibits an otherwise regular apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Each individual populace to expose morphologically unique features. These insights couldn't have been obtained from multiplex staining alone.
Whether you’re a novice or an authority, investigate our sources to advance your exploration and skills in flow cytometry.
(B) RAW 264.7 macrophages in many cases are annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The number of singlets obtainable for analysis boosts as soon as the consumer trains and employs a far more precise product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing which has a large-pace brightfield digital camera for simultaneous substantial throughput flow cytometry and significant resolution brightfield imaging.
"An image is not pretty truly worth 1000 dots, however it seriously really helps to see photographs. It is great to see photos that correlate with staining. For example, AnnexinV-constructive cells which are morphologically distinctive than healthy cells, or CD14+ monocytes which might be larger sized and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it can be illuminating to view how many doublets slip as a result of regular singlet gates, and excellent to verify the standing of unanticipated double-beneficial activities in my Evaluation."
Pair your Attune flow cytometer with the autosampler for amplified workflow effectiveness, and incorporate robotic integration for max throughput.
Consistent picture top quality even at higher throughput Acoustic concentrating and a significant-velocity camera Incorporate to image these T cells continually at lower or substantial flow costs. Quickly regulate target and camera configurations to meet experimental specifications.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can rely on for reliable and trustworthy overall performance. The Attune CytPix design includes a significant-speed brightfield camera and AI-pushed graphic Examination computer software, with latest upgrades together with device Discovering applications and an intuitive consumer interface, enabling enhanced knowledge precision, accuracy, and speedier insights into sample biology.
Possibly most importantly, these occasions have cells of Plainly various phenotypes which could lead to incorrect conclusions concerning double favourable situations (especially in uncommon populations).
Seem waves enable specific mobile alignment, leading to Excellent details high quality and ~10X a lot quicker sample flow premiums.
Application Be aware: Automatic graphic Investigation lessens consumer-to-consumer variability in flow cytometry gating procedures
Leverage the strength of State-of-the-art engineering and innovation to speed up your discoveries. Trust during the Attune Flow Cytometer relatives to deliver the trustworthiness, pace, and accuracy that established your operate apart.
Flexible payment approach from Agilent Financial Alternatives enable click here you to obtain the significant instrumentation you'll need for rapid venture, or to develop your lab.
Gating on these extended parameters allows you to speedily and properly identify populations of curiosity and ensure gating technique with little or no manual evaluate. The Attune application gives use of condition-of-the-art device Discovering Evaluation applications in an expertly intended person interface, rendering it exceptionally user friendly.
The Attune Xenith cytometer is usually a higher-parameter spectral analyzer, supplying the two spectral unmixing and traditional flow abilities for extensive and flexible mobile Examination.
Agilent Price Assure guarantees you at the least 10 years of instrument use from the date you purchase. Normally, We are going to credit score you Using the residual value of the system toward an upgraded model.
Here, we display that by gating methods making use of picture-centered quantitative parameters can distinguish interacting cells from coincident events to more accurately examine interacting cells.